Nothing specific about S2 cells to contribute... However, S2s usually
grow fine in other insect media, such as those intended for lepidopteran
cell lines like the Sf9 and High Five. Protocols established for those
cell lines, for example, check out those using the medium ESF-921, would
probably work for you. Please let us know what works out.
Engin
On 12/21/16 6:15 PM, Xianchi Dong wrote:
> Dear All,
>
> Does anybody have experience with SetMet protein in S2 cells? What
> kind of reagent should I use and is anything else I should pay
> attention to?
> Thanks in advance.
>
> Best,
> Xianchi
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