Dear Andrew,
did you remove all cysteins, and all methionines with the mutations? Your
resolution is about 2A, if I understand correctly. This may be suitable for S-
SAD. I would try to get access to a modern inhouse machine to get high quality
data at high multiplicity. Some modern synchrotron beamlines offer more than
1-circle goniometers, but good quality data is more easily collected with a
multi-circle instrument.
Best,
Tim
On Monday, December 19, 2016 4:42:00 PM CET Andrew Lovering wrote:
> Thanks again Herman,
>
> The protein is a two domain protein (approx 40aa, 350aa split) - searching
> with either is proving fruitless.
>
> Original, wild-type cell = 49 x 75 x 80 P212121
> This painful mutant = 39 x 157 x 75 beta=98.26 P21
>
> (so one can say that there seems to be a relationship there, wt b = mutant
> c, wt c = 2x mutant a?)
>
> We have been bitten in the past by crystallizing contaminants, but (touch
> wood) those are always small crystals one / drop, in a few conditions. This
> problem set has been replicated across at least 6 differing crystals (grown
> in different conditions), where there are many crystals / drop......along
> with the similar cell I'm confident that we are seeing diffraction from
> what we expect
>
> I will check the diffraction images more closely, but (does anyone agree
> here?) I find this sometimes obscured by modern fine-slice/Pilatus
> methodology
>
> Might be one for 2017! p.s. no anomalous signal in there - ironically mutant
> we're using knocks bound metal out!
>
> Thanks again
> Andy
> From: [log in to unmask] [mailto:[log in to unmask]]
> Sent: 19 December 2016 16:26
> To: Andrew Lovering; [log in to unmask]
> Subject: AW: unusual monoclinic relation?
>
> Dear Andy,
>
> I don't think you will solve this pre-Xmas, but here are some more
> suggestions: -is there any relationship with the unit cell of the parent,
> unmutated protein? This might give some ideas of the packing of the problem
> crystals. -are some promising solutions being rejected due to clashes? In
> that case you might try to allow for more clashes. -can the protein be
> split in some separate domains? In that case you could try MR with the
> separate domains. -Are you sure the crystallized protein is the protein you
> think you crystallized? (see contamination database). -Check your
> diffraction images to make sure there are no pathologies present.
>
> Best,
> Herman
>
>
> Von: Andrew Lovering [mailto:[log in to unmask]]
> Gesendet: Montag, 19. Dezember 2016 12:30
> An: Schreuder, Herman R&D/DE;
> [log in to unmask]<mailto:[log in to unmask]> Betreff: RE: unusual
> monoclinic relation?
>
> Thanks Herman. In short:
>
> -no twinning suggested from xtriage etc
> -P2 doesn't give a solution either
> -monoclinic cell would have 2 (60% solvent) or 3 (40% solvent) copies
> -I originally thought the zanuda P1 route would be the way to go, but phaser
> was still churning away after running overnight and so I killed the job
> thinking it was thrashing
>
> Andy
>
> From: [log in to unmask]<mailto:[log in to unmask]>
> [mailto:[log in to unmask]] Sent: 19 December 2016 10:43
> To: Andrew Lovering; [log in to unmask]<mailto:[log in to unmask]>
> Subject: AW: unusual monoclinic relation?
>
> Dear Andrew,
>
> Just a few questions:
> -Do the processing/refinement programs suggest twinning?
> -Are you sure your space group is P21 and not P2? Did you try MR in P2?
> -How many protein molecules do you expect in the asymmetric unit?
>
> P2(1) is a very low symmetry space group. In this case I would not try to be
> clever and just reprocess the data in P1 and run MR in P1. With Zanuda you
> can afterwards try to figure out what the real space group could be.
>
> Best,
> Herman
>
>
> Von: CCP4 bulletin board [mailto:[log in to unmask]] Im Auftrag von
> Andrew Lovering Gesendet: Montag, 19. Dezember 2016 09:43
> An: [log in to unmask]<mailto:[log in to unmask]>
> Betreff: [ccp4bb] unusual monoclinic relation?
>
> Dear All,
>
> I have just collected data on a mutant of a protein that should be facile to
> solve by molrep (one residue/320 changed, approx 2Ang resolution) but is
> proving problematic. Data merging stats look good.
>
> The spacegroup is monoclinic, P21, the cell:
>
> a=39.47 b=157.36 c=74.9 beta=98.26
>
> I spotted the relevant monoclinic twin laws on ccp4 twinning page and all
> relate multiples of axes a and c with one another (na +nc etc) but in the
> above case it would appear b~ = 4a
>
> There are other datasets, all index in this way, some hint at issues by
> indexing with the alternate a=74 b=157 c=79 (where a and c "swap" with a
> doubled, and thus our b=4a turns into b=2c)
>
> I would appreciate any advice on how to progress! Be nice to solve it
> pre-xmas.....
>
> Best & thanks in advance,
> Andy
--
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Paul Scherrer Institut
Tim Gruene
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