Dear FSL experts,
I am working on a TBSS analysis (40 patients and 20 healthy controls).
I followed the steps included in FSL wiki
For "TBSS_3_postreg" when I use the flag "-T" I get results of multiple significant clusters.
When I use "TBSS_3_postreg" with the flag "-S" including a mean template built from all the subjects. I get global effect (one big significant cluster at a very small p value). The global effect is exist if I permute 5000 or 500.
The mean template was created by registering all the FA maps for all the subjects to the best FA map that I have then using the command :
fslmaths image1 -add image2 -add image3 -add ..... -div N template -odt float
In this case which flag (-n or -T) I must use with the script "TBSS_3_postreg "?
I tried to investigate global effect by dividing the images into two groups depending on age and the global effect persists when I used the flag -S with the script TBSS_3_postreg
I highly appreciate any advice