Benzopinacol? It's sometimes used as the initiator for radical
polymerisations, so its presence in plasticware wouldn't be unheard of.
On 2016-11-27 10:33, Wei Liu wrote:
> Dear all,
>
> We have recently crystallized a recombinant protein produce from E.
> coli, and determined the structure at 1.9 Å. The asymmetric unit
> contains two protein monomers. Beyond our expectation, strong Fo - Fc
> density is present at a cleft of one subunit, but not in the other.
> Density maps are given in the snapshots attached to this email. We
> tried to model Tris or Bis-tris propane that were used as the
> purification and crystallization buffers, but apparently either of
> them poorly fitted in this density. The molecule that can be modeled
> here seems more likely to be a ligand comprising 3 or 4 rings with
> good planarity, however we did not add any additives in our
> crystallization trials. So we think it should be something from E.
> coli, which has high affinity to our protein. I wonder if anybody can
> figure out which molecule well fits to the electron density.
>
> Best wishes
> Wei Liu
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