Dear all,
I am trying to solve the structure of a collagen triple helix using phaser. The crystal diffracts to 2.0 A in P1 space group. Matthew's coefficient suggests 4 molecules in the asu. When I tried to search for 5 components, phaser gave 8 solutions each containing 4 triple helices. The LLG scores are in the range of 2200 and TFZ around 15.
All 8 solutions are similar except that the triple helices slide either up or down with respect to each other in different solutions.
Refinement of the top solution using default settings in phenix resulted in the following statistics.
start final
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R-work: 0.5000 0.4003
R-free: 0.4989 0.4586
RMS(angles): 1.82 1.74
RMS(bonds): 0.013 0.009
I am relatively new to crystallography and would appreciate any help in understanding why I am getting multiple solutions and if there is a way to reconcile the results into a single solution.
Thank you
Abhishek
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