Dear Hesna,
If I understand correctly, you’re saying that automated assignment wasn’t able to assign all your Ca and Cb. I suggest you go through them to check the assignments, and assign manually any missing Ca or Cb. You could try the semi-automated procedure described here:
http://www.protein-nmr.org.uk/solution-nmr/assignment-practise/triple-resonance-backbone-assignment/triple-resonance-backbone-assignment-semi-automated/
Best wishes,
Tharin
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Dr. Tharin Blumenschein, Lecturer
School of Chemistry
University of East Anglia
Norwich - NR4 7TJ
United Kingdom
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On 23/08/2016 17:11, "CcpNmr software mailing list on behalf of Hesna KARA" <[log in to unmask] on behalf of [log in to unmask]> wrote:
Hello,
I am using the automated sequence assignment in CCPNMR , so I did my peak picking and transfered all the peaks from the 2D trosy into the 3D HNCA and CBCACONH , and I asked CCPNMR to find inter and intra peaks. after the calculation, I have a big list of Ca and Cb couples. the probleme is that for some residues (spin systems) I don't have Ca and/or Cb and I have to find them and add them to the spin system, to start the Nexus calculation.
Can someone tell me please , how to add (assign ) Ca and Cb to the spin system without Ca and Cb ?
Thank you by advance
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