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CCP4BB  August 2016

CCP4BB August 2016

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Subject:

Re: harvest crystals in isopropanol

From:

"Mitchell D. Miller" <[log in to unmask]>

Reply-To:

Mitchell D. Miller

Date:

Thu, 4 Aug 2016 13:33:49 -0500

Content-Type:

text/plain

Parts/Attachments:

Parts/Attachments

text/plain (166 lines)

Just make sure you don't transport anything that should be
classified as "hazardous".

The Univ. Wisconsin-Madison was fined $56,000 for a student
traveling with 1.9 L of ethyl alcohol and 120 ml of epoxy
hardener in their checked luggage.
http://www.wkow.com/story/31380773/2016/03/03/uw-madison-fined-56000-in-airport-hazmat-incident
https://aps-user-news.aps.anl.gov/072016/hazardous-materials-should-not-be-transported-luggage
The final fine of $56,000 was less than the $70,050 originally
proposed by the FAA.
https://www.faa.gov/news/press_releases/news_story.cfm?newsId=18954

The quantity of the material is important in the hazard reporting
requirements since there are exceptions for de minimis quantities
and less stringent requirements for small quantities. The UW-Madison
case referred to above was in excess of these exceptions limits. See
http://www.jhsph.edu/research/centers-and-institutes/johns-hopkins-education-and-research-center-for-occupational-safety-and-health/2012pdc_slides/Fowlkes_Hazmat%20Presentation.pdf
for a nice overview of some of the issues with the transportation of
hazardous materials.

Regards,
Mitch


Quoting Harry Powell <[log in to unmask]>:

> Hi Nukri
>
> Absolutely - security was very different in those days (and I  
> remember having problems even then), but just because something is  
> hard does not mean it should not be attempted. JFK had something to  
> say on this subject (... not because they are easy, but because...).
>
> Harry
> --
> Dr Harry Powell
> Chairman of International Union of Crystallography Commission on  
> Crystallographic Computing
> Chairman of European Crystallographic Association SIG9  
> (Crystallographic Computing)
>
>> On 4 Aug 2016, at 14:43, Sanishvili, Ruslan <[log in to unmask]> wrote:
>>
>> Hi Harry,
>>
>> I agree with you and others that testing crystals in-situ, at least  
>> for quality if not for full data collection is very useful and wise  
>> thing to do.
>> But I must point out that airport security was very different when  
>> "dry shippers were used for bovine genetic samples" (I'll buy you  
>> beer for the story).
>>
>> For those who want to try, it is often helpful to have some sort of  
>> letter, with official department seal and signature, explaining  
>> what one carries in those beer coolers. And keep in mind that it is  
>> the seal and the signature that are important here, not so much the  
>> content of the letter.
>> Cheers,
>> N.
>>
>> Ruslan Sanishvili (Nukri), Ph.D.
>> Macromolecular Crystallographer
>> GM/CA@APS
>> X-ray Science Division, ANL
>> 9700 S. Cass Ave.
>> Lemont, IL 60439
>>
>> Tel: (630)252-0665
>> Fax: (630)252-0667
>> [log in to unmask]
>>
>> From: CCP4 bulletin board [[log in to unmask]] on behalf of  
>> Harry Powell [[log in to unmask]]
>> Sent: Thursday, August 04, 2016 4:06 AM
>> To: [log in to unmask]
>> Subject: Re: [ccp4bb] harvest crystals in isopropanol
>>
>> Hi
>>
>> Just to reinforce Mark's point, in the days before cryocooling was  
>> the norm (and dry shippers were used for bovine genetic samples  
>> [buy me a beer sometime and I'll bore you with the story]), we  
>> always used to carry our crystals in (at least) two ways to the  
>> synchrotron (through security and with us on the 'plane).
>>
>> We always took some crystals mounted in capillaries, but also took  
>> some of our crystallisation plates as well, with "spare" crystals  
>> just in case we had time or needed them. We carried them in very  
>> handy 4-can beer coolers to try to avoid thermal shock - you can  
>> open these and show the security people what you're actually  
>> carrying, and you can keep them upright as well).
>>
>> It may even be possible to set up crystallisation in a lab at your  
>> favourite synchrotron, so the in-situ crystals are waiting for your  
>> arrival to carry out the data collection.
>>
>>> On 4 Aug 2016, at 09:27, Mark J van Raaij wrote:
>>>
>>> perhaps what your crystals need is more important than what you need…
>>>
>>> In-plate diffraction is the only way for you to find out how well  
>>> these crystals diffract before doing anything to them. If in the  
>>> plate they still diffract poorly, your efforts may be better  
>>> directed at getting a different crystal form.
>>> If in the plate they diffract ok, then you are more justified to  
>>> spend a lot of time in finding a good cryo-protection protocol  
>>> (btw I don’t think there is a general protocol, you’ll have to get  
>>> a feel for your crystals and what they like).
>>>
>>> Mark J van Raaij
>>> Dpto de Estructura de Macromoleculas
>>> Centro Nacional de Biotecnologia - CSIC
>>> c/Darwin 3
>>> E-28049 Madrid, Spain
>>> tel. (+34) 91 585 4616
>>> http://wwwuser.cnb.csic.es/~mjvanraaij
>>>
>>>
>>>
>>>
>>>
>>>
>>>>> On 4 Aug 2016, at 04:56, 张士军 <[log in to unmask]> wrote:
>>>> NO ,I need stored in LN2 first ,then  transport to synchron to diffract
>>>> 在2016-08-04 10:46:42,张士军[log in to unmask]写道:
>>>> In situ diffraction?
>>>> JPK
>>>> From: CCP4 bulletin board [mailto:[log in to unmask]] On Behalf Of ???
>>>> Sent: Wednesday, August 03, 2016 9:20 PM
>>>> To: [log in to unmask]
>>>> Subject: [ccp4bb] harvest crystals in isopropanol
>>>> Hi all
>>>>   I have a crystal grown in 35%isopropanol ,5%PEG1000/8000,pH5.5  
>>>> .Because isopropanol is very easy to volatilize ,so it hard to  
>>>> harvest ,and it's easy to damage the crystals. Can anyone give me  
>>>> some suggestions about how to harvest this kind of crystals,or  
>>>> the detail process and buffer to harvest them .Thanks a lot !!!!!
>>>>                                                                    
>>>>                                                                    
>>>>   ShijunZhang
>>>>                                                                    
>>>>                                                     School of  
>>>> Life Science
>>>>                                                                    
>>>>                                                          Xiamen  
>>>> University
>>
>> Harry
>> --
>> Dr Harry Powell
>> Chairman of International Union of Crystallography Commission on  
>> Crystallographic Computing
>> Chairman of European Crystallographic Association SIG9  
>> (Crystallographic Computing)
>>
>>
>>
>>
>>
>>
>>
>>
>>
>>
>>

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