perhaps what your crystals need is more important than what you need…
In-plate diffraction is the only way for you to find out how well these crystals diffract before doing anything to them. If in the plate they still diffract poorly, your efforts may be better directed at getting a different crystal form.
If in the plate they diffract ok, then you are more justified to spend a lot of time in finding a good cryo-protection protocol (btw I don’t think there is a general protocol, you’ll have to get a feel for your crystals and what they like).
Mark J van Raaij
Dpto de Estructura de Macromoleculas
Centro Nacional de Biotecnologia - CSIC
c/Darwin 3
E-28049 Madrid, Spain
tel. (+34) 91 585 4616
http://wwwuser.cnb.csic.es/~mjvanraaij
> On 4 Aug 2016, at 04:56, 张士军 <[log in to unmask]> wrote:
>
> NO ,I need stored in LN2 first ,then transport to synchron to diffract
> 在2016-08-04 10:46:42,张士军[log in to unmask]写道:
>
> In situ diffraction?
>
>
>
> JPK
>
>
>
> From: CCP4 bulletin board [mailto:[log in to unmask]] On Behalf Of ???
> Sent: Wednesday, August 03, 2016 9:20 PM
> To: [log in to unmask]
> Subject: [ccp4bb] harvest crystals in isopropanol
>
>
>
> Hi all
> I have a crystal grown in 35%isopropanol ,5%PEG1000/8000,pH5.5 .Because isopropanol is very easy to volatilize ,so it hard to harvest ,and it's easy to damage the crystals. Can anyone give me some suggestions about how to harvest this kind of crystals,or the detail process and buffer to harvest them .Thanks a lot !!!!!
> ShijunZhang
> School of Life Science
> Xiamen University
|