Dear Nicolas,
No problem for further questions!! Well, I use Refmac for structure
refinement and I have used this software many times without any problem
up to now. I have already added the ligand, ions and the majority of
water molecules into the structure.
The issue of data quality is important because it should be obvious to
obtain good electron density maps and good R and Rfree with good data
(high resolution, good completeness and so on), but in the case of DNA,
or at least this kind of DNA folding (G-quadruplex), this may not
happen. I haven't used TLS yet.
Thank you
Francesco Papi
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