Hi All,
I have been obtaining a multiple thin plates crystals attached to each other on the surface of the sitting drop. I have tried additive and silver bullet screen from Hampton to see if they improve, but all my hits in the new screen are again almost the same. pH optimization around the initial hit (5.5 and 6.5) also gives similar crystals within the range. Also the rMMS method gave crystals of similar morphology on several wells. I would appreciate your tips on improving this crystal.
P.S. the protein is ~50 kDa, pI 9.6, and has 20 mM MOPS and 400 mM KCL in the storage buffer (pH 7.0). Protein concentration used in this experiment is 9.0 mg/mL
Thank you,
Bishwa
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