Hi all,
I am currently assigning an intrinsically disordered protein which shows significant signal overlap. In a few cases,HSQC peaks can not be resolved at all. Thus, in the 3D experiments I observe strips with multiple peaks, corresponding to 2 different residues, at the same H-N frequencies.
My question is about how to handle this consistently during the assignment from root: shall I create two separate root peaks, virtually at the same position, each one with its own resonances and spin systems and try to assign them in latter stages; or either a single peak with multiple resonances? Maybe there is another (more proper) way to do it?
Thank you in advance and congratulations for your work and support!
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