Hi Amu,
Great that your problem has been solved! May I remind you (and the
list), that the tutorial on the RELION Wiki
(http://www2.mrc-lmb.cam.ac.uk/groups/scheres/relion13_tutorial.pdf) is
the best way to learn how to use RELION. For example, on page 10 it had
the answer to your question.
Best wishes,
Sjors
On 05/19/2016 02:35 PM, Amrita Samanta wrote:
> Hi CCPEM members,
>
> Thank you for your help. It got fixed after I did contrast inversion.
>
> Thanks
> Amu
>
> On Wed, May 18, 2016 at 6:37 PM, Sudheer Molugu <[log in to unmask]> wrote:
>
>> Did it fix? I can help you. Let me know. Thanks.
>> Sudheer.
>>
>>
>> On Wednesday, May 18, 2016, Amrita Samanta <[log in to unmask]> wrote:
>>
>>> Hi,
>>>
>>> I picked the particles using EMAN2 but when I used those .box files for
>>> 2D classification they looked black. I tried phase flipping and still they
>>> look black. What can I do to make the particles look white.
>>>
>>> Thank you
>>> Amu
>>>
>>
>> --
>> Sent from Gmail Mobile
>>
--
Sjors Scheres
MRC Laboratory of Molecular Biology
Francis Crick Avenue, Cambridge Biomedical Campus
Cambridge CB2 0QH, U.K.
tel: +44 (0)1223 267061
http://www2.mrc-lmb.cam.ac.uk/groups/scheres
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