Dear Prem,
You probably made an error in combining STAR files from the different
classes, as the rlnRandomSubset is not always 1 or 2. Be aware that column
numbering may be different in different STAR files: the header defines the
order of the columns!
HTH,
Sjors

> Dear all,
>
> I am refining a data set after successful 2D and 3D Classification in
> relion 1.4. my input script is
>
> mpirun -pernode -n 30 `which relion_refine_mpi` --o Ref-class-1-2-3/run1
> --auto_refine --split_random_halves --i Class-1-2-3-merg.star
> --particle_diameter 430 --angpix 1.25 --ref run3_6.0A_class001.mrc
> --ini_high 50 --ctf --ctf_corrected_ref --flatten_solvent --zero_mask
> --oversampling 1 --healpix_order 2 --auto_local_healpix_order 4
> --offset_range 5 --offset_step 2 --sym C1 --low_resol_join_halves 40
> --norm --scale --j 8 --memory_per_thread 4
> --------------
> it is giving following error
> -------------------
> Warning: no access to tty (Bad file descriptor).
> Thus no job control in this shell.
> /usr15/data/psk01/DATA1
> === RELION MPI setup ===
> + Number of MPI processes = 30
> + Number of threads per MPI process = 8
> + Total number of threads therefore = 240
> + Master (0) runs on host = node54
> + Slave 1 runs on host = node53
> + Slave 2 runs on host = node52
> + Slave 3 runs on host = node51
> + Slave 4 runs on host = node50
> + Slave 5 runs on host = node49
> + Slave 6 runs on host = node48
> + Slave 7 runs on host = node47
> + Slave 8 runs on host = node46
> + Slave 9 runs on host = node45
> + Slave 10 runs on host = node44
> + Slave 11 runs on host = node43
> + Slave 12 runs on host = node42
> + Slave 13 runs on host = node41
> + Slave 14 runs on host = node40
> + Slave 15 runs on host = node39
> + Slave 16 runs on host = node38
> + Slave 17 runs on host = node37
> + Slave 18 runs on host = node36
> + Slave 19 runs on host = node35
> + Slave 20 runs on host = node34
> + Slave 21 runs on host = node33
> + Slave 22 runs on host = node32
> + Slave 23 runs on host = node31
> + Slave 24 runs on host = node30
> + Slave 25 runs on host = node29
> + Slave 26 runs on host = node28
> + Slave 27 runs on host = node27
> + Slave 28 runs on host = node26
> + Slave 29 runs on host = node25
> =================
> Running in double precision.
> ERROR Experiment::divideParticlesInRandomHalves: invalid number for random
> subset (i.e. not 1 or 2): 7
> File: src/exp_model.cpp line: 211
> --------------------------------------------------------------------------
> MPI_ABORT was invoked on rank 5 in communicator MPI_COMM_WORLD
> with errorcode 1.
>
> NOTE: invoking MPI_ABORT causes Open MPI to kill all MPI processes.
> You may or may not see output from other processes, depending on
> exactly when Open MPI kills them.
> --------------------------------------------------------------------------
> ERROR Experiment::divideParticlesInRandomHalves: invalid number for random
> subset (i.e. not 1 or 2): 7
> File: src/exp_model.cpp line: 211
> ERROR Experiment::divideParticlesInRandomHalves: invalid number for random
> subset (i.e. not 1 or 2): 7
> File: src/exp_model.cpp line: 211
> [node54:19017] 1 more process has sent help message help-mpi-api.txt /
> mpi-abort
> [node54:19017] Set MCA parameter "orte_base_help_aggregate" to 0 to see
> all help / error messages
> ---------------------
> Your advice and suggestion would be greatly appreciated
>
> with regards
> -Prem
>
>


--
Sjors Scheres
MRC Laboratory of Molecular Biology
Francis Crick Avenue, Cambridge Biomedical Campus
Cambridge CB2 0QH, U.K.
tel: +44 (0)1223 267061
http://www2.mrc-lmb.cam.ac.uk/groups/scheres