Check the codon usage database:
http://www.kazusa.or.jp/codon/
for Spodoptera frugiperda:
http://www.kazusa.or.jp/codon/cgi-bin/showcodon.cgi?species=10455
Mark J van Raaij
Dpto de Estructura de Macromoleculas
Centro Nacional de Biotecnologia - CSIC
c/Darwin 3
E-28049 Madrid, Spain
tel. (+34) 91 585 4616
http://wwwuser.cnb.csic.es/~mjvanraaij
> On 16 Feb 2016, at 14:47, Reza Khayat <[log in to unmask]> wrote:
>
> Hi,
>
> We have an E. coli codon optimized gene that we would like to try expressing in insect cells. The protein is not expressing and we are concerned that it may be because of the codon optimization. Could this be a possibility, or are the insect optimized codons similar to that of E. coli? Thanks.
>
> Best wishes,
> Reza
>
> Reza Khayat, PhD
> Assistant Professor
> City College of New York
> Department of Chemistry
> New York, NY 10031
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