I have done particle extraction from movies containing 68 frames (4s total exposure, 17 frames/s) without averaging, but the initial Refine3D (movie expansion) crushes.
I have checked every movie using bhead and all of them contain at least 68 frames (2 of them contain 70). And movies have the same size except one that is removed from the starting files.
I plan to redo the particle extraction from frames. In order to decrease file sizes and RAM request, would you recommend me to average frames during the particle extraction (for example every 3 frames) or I better work with frames 1 to 20?
Thank you very much in advance.