Chen,
I've recently begun a similar pursuit. These two papers have been my
guide in the last few weeks:
Dong et al (2007) describe their application of in situ proteolysis to
a number of different human or bacterial proteins, many of which had
not otherwise been crystallized:
http://www.nature.com/nmeth/journal/v4/n12/abs/nmeth1118.html; and
McPherson et al (2004) tell eloquently their fortuitous success using
in situ proteolysis to crystallize one protein in particular that had
otherwise not crystallized, or crystallized only over an extremely
long time period. A long time to crystal formation may be an indicator
of proteolysis-driven crystallization:
http://scripts.iucr.org/cgi-bin/paper?S0907444904005025
Wish you well,
Emily.
On Thu, Jan 14, 2016 at 6:45 AM, <Xia Chen> <[log in to unmask]> wrote:
> Now, my protein is resist to crystal. so I want to do limited-proteolysis. though I can get some hit from the pubmed paper. but it has not detail. so is there anybody has the some mannuals or guide about it. can you send it to me.
>
> Chen Xia, Ph.D
>
> School of Life Sciences
> Tianjin University
> 92 Weijin Road, Nankai District, Tianjin 300072, P.R.China
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