Dear Maciej,
Either some file got corrupted, or you did (unwillingly) change some of
the stacks at some point. You have pointers in them that are too large for
the stacks, e.g. [log in to unmask], where somestack.mrcs only contains 88
images.
HTH,
Sjors
> I ran 2D classification (Relion-1.4) which finished with no problems. I
> attempted to start a second round of 2D and got the errors
>
> In thread 0
> readMRC: Image number 560 exceeds stack size 88
> File: ./src/rwMRC.h line: 193
> readMRC: Image number 547 exceeds stack size 208
> File: ./src/rwMRC.h line: 193
> readMRC: Image number 524 exceeds stack size 497
> File: ./src/rwMRC.h line: 193
> readMRC: Image number 351 exceeds stack size 1
> File: ./src/rwMRC.h line: 193
> readMRC: Image number 510 exceeds stack size 387
> File: ./src/rwMRC.h line: 193
> readMRC: Image number 35 exceeds stack size 1
> File: ./src/rwMRC.h line: 193
> readMRC: Image number 456 exceeds stack size 1
> File: ./src/rwMRC.h line: 193
> readMRC: Image number 372 exceeds stack size 88
> File: ./src/rwMRC.h line: 193
> readMRC: Image number 409 exceeds stack size 367
> File: ./src/rwMRC.h line: 193
> readMRC: Image number 76 exceeds stack size 1
>
>
> There were no changes to the data from the first 2D run (except saving
> particles from a subset of the classes from the first run). To
> troubleshoot I tried continuing the first run with an additional
> iteration, so that everything would be the same. I still got the stack
> size error. I used 1.4 a lot during the past weeks and have never seen
> this problem.
>
> I also can not repeat previously successful relion-1.4 operations (e.g.,
> the particle extraction job that ran fine previously now fails with a
> related error ./src/rwMRC.h problem with image header)
>
> Assistance will be appreciated.
> Maciej
>
--
Sjors Scheres
MRC Laboratory of Molecular Biology
Francis Crick Avenue, Cambridge Biomedical Campus
Cambridge CB2 0QH, U.K.
tel: +44 (0)1223 267061
http://www2.mrc-lmb.cam.ac.uk/groups/scheres
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