Hi!!!
Relion tutorials and protocols always recommend normalize with a
reasonable radius for the circle around your particle. After particle
extraction, I have seen a huge differences using smaller bg_radius. If I
select a big bg_radius that cover the total box size then 2d
classification looks nicer.
Could I ask if make sense to normalize with relion_preprocess with a
same size bg_radius as the box size?
Thank you very much.
Best
Hugo
--
Hugo Muñoz Hernández, PhD student
Centro de Investigaciones Biológicas CIB-CSIC
Consejo Superior de Investigaciones Científicas
C/ Ramiro de Maeztu, 9
28040 Madrid (Spain)
http://www.cib.csic.es/es/grupo.php?idgrupo=47
Tel: +34 91 8373112 ext 4436, Lab.B-47
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