Hello,

I have been playing around with relion_project script. I am able to generate 1 back-projection at a time at given angles (arguments --rot, --tilt, --psi). 
I could not execute the script with argument '--ang' which seems to be the key when attempting to generate a series of back-projections. What kind of file do I need to add to the '--ang' argument to make the script generate such a series? Input format says a .star file, but I had no success using .star files with 2d or 3d classes.  

The second part of my question is this: does finding the best matching 2D classes and re-projections means that is done just by eye? 

Regards,
Maciej

On Fri, 20 Nov 2015 17:35:27 +0000, Sjors Scheres <[log in to unmask]> wrote:

>Hi Maciej,
>relion_project can make projections of your model. Type the programname
>without options on the command line to get help.
>You'll have to find the best-matching classes and re-projections yourself.
>HTH,
>Sjors
>
>On 11/20/2015 04:11 PM, Maciej Jagielnicki wrote:
>> Hello,
>>
>> I am in the point of generating figures for my 3D refined map.
>>
>> I am looking for a way to generate 2D projections (back projections) from my final 3D refined map and then matching those 2D projections with 2D class averages (i.e. generating 2D projections at certain euler angles and then also showing 2d class averages at the same euler angles).
>>
>> Ideally, a figure would have a set of 1) 2D projections of a 3D refined map followed by 2) corresponding 2D class averages followed by 3) particles representing each 2D class average.
>>
>> I would greatly appreciate any suggestions you might have.
>>
>> Regards,
>> Maciej Jagielnicki
>> University of Virginia
>
>--
>Sjors Scheres
>MRC Laboratory of Molecular Biology
>Francis Crick Avenue, Cambridge Biomedical Campus
>Cambridge CB2 0QH, U.K.
>tel: +44 (0)1223 267061
>http://www2.mrc-lmb.cam.ac.uk/groups/scheres