Hi Abid and Elena,
If your angles come from another program, you'll have to make a
RELION-type STAR file with the right angles (in
SPIDER/XMIPP/FREALIGN/RELION convention) and all the CTF information
etc. This is usually not trivial, but can be achieved with scripting.
Perhaps the following (now rather old) WIki page will help:
http://www2.mrc-lmb.cam.ac.uk/relion/index.php/Prepare_input_files
Please do note that often it is _much_ easier to re-extract particles
(and re-estimate all CTFs) in RELION to generate the particle STAR file.
You could then use your current model to align against and thus get
orientations within the RELION framework. This is much less prone to
errors, and therefore by far recommended. It also prevents suboptimal
refinements starting from "CTF-corrected" particles (which is a somewhat
alien concept in RELION).
HTH,
Sjors
> On 20/10/2015 15:47, Abid Javed wrote:
>> Dear Scheres,
>>
>> I hope this finds you well.
>>
>> I have a dilemma that I'd like to resolve with your help.
>> I have a stack of cryo-EM images (single particles) and an initial
>> model (both having CTF corrected) and I'd like to sort out
>> heterogeneity in my data set by running 3D classification in RELION.
>>
>> Is there a way I can input my stack of particles that have undergone
>> alignment already and use the 3D classification programme in RELION?
>>
>> Many thanks for your help.
>> Best wishes,
>> Abid.
>
>
--
Sjors Scheres
MRC Laboratory of Molecular Biology
Francis Crick Avenue, Cambridge Biomedical Campus
Cambridge CB2 0QH, U.K.
tel: +44 (0)1223 267061
http://www2.mrc-lmb.cam.ac.uk/groups/scheres
|