Thanks Sjors :-)
Le 14.10.15 10:02, « Sjors Scheres » <[log in to unmask]> a écrit :
>Hi Ben,
>relion_image_handler --i in.mrc --o out.mrc --bfactor -500 --lowpass 4
>--angpix 1.4
>HTH,
>Sjors
>
>> Hi Yan,
>>
>> You can also take a look into the Bsoft package.
>> The program blocres outputs a map of local resolution, similarly to
>> ResMap.
>> You feed the blocfilter with your cryoEM map and your local resolution
>> map. The program does local filtering, and the local resolution cutoffs
>> are based on your resolution map.
>> I must admit that although we have obtained good resolution maps with
>> blocres we have not played much at all with blocfilter, so I cannot
>> comment how well it performs.
>>
>> BTW Sjors, if you do your model building on a half map, how do you
>>sharpen
>> your half map? We couldn¹t find how to do it in relion, so we used
>>bfilter
>> from Bsoft to apply on each half maps the B-factor value obtained during
>> relion post processing, which worked well.
>>
>> Best
>> Benoit
>> Le 13.10.15 20:59, « Collaborative Computational Project in Electron
>> cryo-Microscopy on behalf of Sjors Scheres » <[log in to unmask] on
>> behalf of [log in to unmask]> a écrit :
>>
>>>Hi Yan,
>>>We typically use many different B-factors and (adhoc) lowpass filters in
>>>the postprocessing to generate a range of maps for model building. I
>>> think
>>>Hemant Tagare has plans to develop a local-filtering approach inside
>>>ResMap, but for the time being many maps is a solution.
>>>HTH,
>>>Sjors
>>>
>>>
>>>> Hi all,
>>>>
>>>> I was wondering is there a way to apply B-factor according to local
>>>> resolution rather than one universal B-factor to whole map during
>>>> post-processing?
>>>>
>>>> Thanks for your attention.
>>>> Yan
>>>>
>>>
>>>
>>>--
>>>Sjors Scheres
>>>MRC Laboratory of Molecular Biology
>>>Francis Crick Avenue, Cambridge Biomedical Campus
>>>Cambridge CB2 0QH, U.K.
>>>tel: +44 (0)1223 267061
>>>http://www2.mrc-lmb.cam.ac.uk/groups/scheres
>>
>>
>
>
>--
>Sjors Scheres
>MRC Laboratory of Molecular Biology
>Francis Crick Avenue, Cambridge Biomedical Campus
>Cambridge CB2 0QH, U.K.
>tel: +44 (0)1223 267061
>http://www2.mrc-lmb.cam.ac.uk/groups/scheres
>
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