Hi Ben,
relion_image_handler --i in.mrc --o out.mrc --bfactor -500 --lowpass 4
--angpix 1.4
HTH,
Sjors
> Hi Yan,
>
> You can also take a look into the Bsoft package.
> The program blocres outputs a map of local resolution, similarly to
> ResMap.
> You feed the blocfilter with your cryoEM map and your local resolution
> map. The program does local filtering, and the local resolution cutoffs
> are based on your resolution map.
> I must admit that although we have obtained good resolution maps with
> blocres we have not played much at all with blocfilter, so I cannot
> comment how well it performs.
>
> BTW Sjors, if you do your model building on a half map, how do you sharpen
> your half map? We couldn¹t find how to do it in relion, so we used bfilter
> from Bsoft to apply on each half maps the B-factor value obtained during
> relion post processing, which worked well.
>
> Best
> Benoit
> Le 13.10.15 20:59, « Collaborative Computational Project in Electron
> cryo-Microscopy on behalf of Sjors Scheres » <[log in to unmask] on
> behalf of [log in to unmask]> a écrit :
>
>>Hi Yan,
>>We typically use many different B-factors and (adhoc) lowpass filters in
>>the postprocessing to generate a range of maps for model building. I
>> think
>>Hemant Tagare has plans to develop a local-filtering approach inside
>>ResMap, but for the time being many maps is a solution.
>>HTH,
>>Sjors
>>
>>
>>> Hi all,
>>>
>>> I was wondering is there a way to apply B-factor according to local
>>> resolution rather than one universal B-factor to whole map during
>>> post-processing?
>>>
>>> Thanks for your attention.
>>> Yan
>>>
>>
>>
>>--
>>Sjors Scheres
>>MRC Laboratory of Molecular Biology
>>Francis Crick Avenue, Cambridge Biomedical Campus
>>Cambridge CB2 0QH, U.K.
>>tel: +44 (0)1223 267061
>>http://www2.mrc-lmb.cam.ac.uk/groups/scheres
>
>
--
Sjors Scheres
MRC Laboratory of Molecular Biology
Francis Crick Avenue, Cambridge Biomedical Campus
Cambridge CB2 0QH, U.K.
tel: +44 (0)1223 267061
http://www2.mrc-lmb.cam.ac.uk/groups/scheres
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