Dear all
I have a low-resolution dataset to about 4.2 A processed with XDS. As all three of the CHI2-VALUE OF FIT OF CORRECTION FACTORS values were about 1.4, I processed the images assuming Friedel's law=false and STRICT_ABSORPTION_CORRECTION=TRUE. However, using Xtriage, the anomalous signal seems to only extend to about 12 A at the most. So, I have two questions:
1. Can I process it by assuming Friedel's law=true?
2. How can I know where the signal comes from? The protein contains Fe (about 11 atoms) and the dataset was not collected at the absorption edge? In fact, the structure could/should be solvable using molecular replacement and I don't intend to do experimental phasing.
I can post the processing stats if necessary. Thanks.
Mohamed
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