Dear Monica,
You could try the HATODAS II heavy atom database detailed here in their paper in J Appl Cryst :-
http://dx.doi.org/10.1107/S0021889809012370
Best wishes,
John
Emeritus Prof John R Helliwell DSc_Physics
School of Chemistry, University of Manchester, M13 9PL, UK.
On 5 Oct 2015, at 08:38, Monica Mittal <[log in to unmask]> wrote:
> Dear all,
>
> I need a general advice. I don't have a suitable Model (Lets say only with a similarity of 15%) and no other model to solve the structure by MR. Then i think of anomalous experiments, but soaking with heavy metals is not good for the crystals. Since the protein is being expressed in Pichia cells, growth in Seleno-media is cumbersome. There are no ligands or ions that i can use for anomalous scattering of protein crystals. I am trying to express it in bacteria but have some issues. What is the option that i can choose to solve the structure of such a protein ? Your suggestions are highly recommended.
>
> Thanks in advance.
> Monica
|