Well put, Graham. I was going to reply similarly, but I think you describe very well the variability in chylomicron particle size and their triglyceride content. As I mentioned previously in this thread, several publications and my own unpublished observations show that there is very little correlation between L (or T)-index and triglyceride concentration in lipemic specimens.
Note that Soha Zouwail mentioned to me off list that she found good correlation between the triglyceride concentration in Intralipid and L-index. I have used Intralipid to check for lipemia (turbidity) interference, but did not look at the correlation with the L-index. In fact, I think the last time I did such interference studies was before the specimen indices era. I think this makes sense though because the lipid vesicles of Intralipid are likely to be much more uniform than chylomicrons.
-Jim
-----Original Message-----
From: Clinical biochemistry discussion list [mailto:[log in to unmask]] On Behalf Of Graham Jones
Sent: Wednesday, September 02, 2015 8:27 AM
To: [log in to unmask]
Subject: Re: Lipaemia index
Derreck,
I expect not. Rather than having a lot of nice fresh chylomicrons, all the same size and same triglycerides (TG) content (and perhaps the same light scattering), I would expect in any sample we may have a range of difference sizes and TG content (and light scattering) as they lose TG to tissues on their way to the liver. Thus the relationship between TG and light scattering is not very close.
Just my thoughts,
Graham
________________________________________
From: Clinical biochemistry discussion list <[log in to unmask]> on behalf of Mccullough Derreck (UHMB) <[log in to unmask]>
Sent: Wednesday, 2 September 2015 6:45 PM
To: [log in to unmask]
Subject: Re: Lipaemia index
Hi Jim,
As chylomicrons are 90% triglyceride would you not expect a good correlation between triglycerides and turbidity if turbidity is caused by chylomicrons?
Bw,
Derreck
-----Original Message-----
From: Clinical biochemistry discussion list [mailto:[log in to unmask]] On Behalf Of [log in to unmask]
Sent: 26 August 2015 18:14
To: [log in to unmask]
Subject: Re: Lipaemia index
Replying to myself here! I agree with Alan that Sonia's triglyceride vs L- (or T-index) is much higher than expected. I've had a chance to dig out one of my correlations: Triglyceride, mg/dL = 0.88 L-index + 185, R^2 = 0.017. (Sorry for U.S. units, but the key here is the very low R^2.) My bad that I didn't indicate the instrument/method on the graph, but it was a Roche instrument, probably an Integra. Also, earlier I implied that VLDL, being triglyceride rich, would increase the L/T-index. VLDL rarely does, but most often it is chylomicrons that cause the turbidity.
Sonia, you mention that your L-index results are binned, that is, 1+, 2+, etc. yet your graph has numbers for the L-index. Does your Architect give you both the numerical L-index and the binned result?
-Jim
------ACB discussion List Information-------- This is an open discussion list for the academic and clinical community working in clinical biochemistry.
Please note, archived messages are public and can be viewed via the internet. Views expressed are those of the individual and they are responsible for all message content.
ACB Web Site
https://urldefense.proofpoint.com/v2/url?u=http-3A__www.acb.org.uk&d=AwIFAg&c=SgMrq23dbjbGX6e0ZsSHgEZX6A4IAf1SO3AJ2bNrHlk&r=_4rCeX3TSQgrdhhwEl4yDs13b8mZhuDoiRSP4oKKuU4&m=AOWGrF-KHy8M7WIW5Km5Z9khIGSuCxq_XCYo4Zjn1Ms&s=ASzVuUmIgFDfaCCPNg6C4bgK3LpvhQhHVI9iLJIaxRU&e=
Green Laboratories Work
https://urldefense.proofpoint.com/v2/url?u=http-3A__www.laboratorymedicine.nhs.uk&d=AwIFAg&c=SgMrq23dbjbGX6e0ZsSHgEZX6A4IAf1SO3AJ2bNrHlk&r=_4rCeX3TSQgrdhhwEl4yDs13b8mZhuDoiRSP4oKKuU4&m=AOWGrF-KHy8M7WIW5Km5Z9khIGSuCxq_XCYo4Zjn1Ms&s=3--mLJT9NarSD613B7aQMMDh0Xri-UDmXRPZ6T9r1rU&e=
List Archives
https://urldefense.proofpoint.com/v2/url?u=http-3A__www.jiscmail.ac.uk_lists_ACB-2DCLIN-2DCHEM-2DGEN.html&d=AwIFAg&c=SgMrq23dbjbGX6e0ZsSHgEZX6A4IAf1SO3AJ2bNrHlk&r=_4rCeX3TSQgrdhhwEl4yDs13b8mZhuDoiRSP4oKKuU4&m=AOWGrF-KHy8M7WIW5Km5Z9khIGSuCxq_XCYo4Zjn1Ms&s=cMxp_NanXU8H1bMHft0ynXNqWBO9iOXZ39XRyBZ8egc&e=
List Instructions (How to leave etc.)
https://urldefense.proofpoint.com/v2/url?u=http-3A__www.jiscmail.ac.uk_&d=AwIFAg&c=SgMrq23dbjbGX6e0ZsSHgEZX6A4IAf1SO3AJ2bNrHlk&r=_4rCeX3TSQgrdhhwEl4yDs13b8mZhuDoiRSP4oKKuU4&m=AOWGrF-KHy8M7WIW5Km5Z9khIGSuCxq_XCYo4Zjn1Ms&s=rZZbQTBaSsZV_YDduI9RBB11sTw04YoR2N1dzUqzMag&e=
------ACB discussion List Information-------- This is an open discussion list for the academic and clinical community working in clinical biochemistry.
Please note, archived messages are public and can be viewed via the internet. Views expressed are those of the individual and they are responsible for all message content.
ACB Web Site
https://urldefense.proofpoint.com/v2/url?u=http-3A__www.acb.org.uk&d=AwIFAg&c=SgMrq23dbjbGX6e0ZsSHgEZX6A4IAf1SO3AJ2bNrHlk&r=_4rCeX3TSQgrdhhwEl4yDs13b8mZhuDoiRSP4oKKuU4&m=AOWGrF-KHy8M7WIW5Km5Z9khIGSuCxq_XCYo4Zjn1Ms&s=ASzVuUmIgFDfaCCPNg6C4bgK3LpvhQhHVI9iLJIaxRU&e=
Green Laboratories Work
https://urldefense.proofpoint.com/v2/url?u=http-3A__www.laboratorymedicine.nhs.uk&d=AwIFAg&c=SgMrq23dbjbGX6e0ZsSHgEZX6A4IAf1SO3AJ2bNrHlk&r=_4rCeX3TSQgrdhhwEl4yDs13b8mZhuDoiRSP4oKKuU4&m=AOWGrF-KHy8M7WIW5Km5Z9khIGSuCxq_XCYo4Zjn1Ms&s=3--mLJT9NarSD613B7aQMMDh0Xri-UDmXRPZ6T9r1rU&e=
List Archives
https://urldefense.proofpoint.com/v2/url?u=http-3A__www.jiscmail.ac.uk_lists_ACB-2DCLIN-2DCHEM-2DGEN.html&d=AwIFAg&c=SgMrq23dbjbGX6e0ZsSHgEZX6A4IAf1SO3AJ2bNrHlk&r=_4rCeX3TSQgrdhhwEl4yDs13b8mZhuDoiRSP4oKKuU4&m=AOWGrF-KHy8M7WIW5Km5Z9khIGSuCxq_XCYo4Zjn1Ms&s=cMxp_NanXU8H1bMHft0ynXNqWBO9iOXZ39XRyBZ8egc&e=
List Instructions (How to leave etc.)
https://urldefense.proofpoint.com/v2/url?u=http-3A__www.jiscmail.ac.uk_&d=AwIFAg&c=SgMrq23dbjbGX6e0ZsSHgEZX6A4IAf1SO3AJ2bNrHlk&r=_4rCeX3TSQgrdhhwEl4yDs13b8mZhuDoiRSP4oKKuU4&m=AOWGrF-KHy8M7WIW5Km5Z9khIGSuCxq_XCYo4Zjn1Ms&s=rZZbQTBaSsZV_YDduI9RBB11sTw04YoR2N1dzUqzMag&e=
------ACB discussion List Information-------- This is an open discussion list for the academic and clinical community working in clinical biochemistry.
Please note, archived messages are public and can be viewed via the internet. Views expressed are those of the individual and they are responsible for all message content.
ACB Web Site
https://urldefense.proofpoint.com/v2/url?u=http-3A__www.acb.org.uk&d=AwIFAg&c=SgMrq23dbjbGX6e0ZsSHgEZX6A4IAf1SO3AJ2bNrHlk&r=_4rCeX3TSQgrdhhwEl4yDs13b8mZhuDoiRSP4oKKuU4&m=AOWGrF-KHy8M7WIW5Km5Z9khIGSuCxq_XCYo4Zjn1Ms&s=ASzVuUmIgFDfaCCPNg6C4bgK3LpvhQhHVI9iLJIaxRU&e=
Green Laboratories Work
https://urldefense.proofpoint.com/v2/url?u=http-3A__www.laboratorymedicine.nhs.uk&d=AwIFAg&c=SgMrq23dbjbGX6e0ZsSHgEZX6A4IAf1SO3AJ2bNrHlk&r=_4rCeX3TSQgrdhhwEl4yDs13b8mZhuDoiRSP4oKKuU4&m=AOWGrF-KHy8M7WIW5Km5Z9khIGSuCxq_XCYo4Zjn1Ms&s=3--mLJT9NarSD613B7aQMMDh0Xri-UDmXRPZ6T9r1rU&e=
List Archives
https://urldefense.proofpoint.com/v2/url?u=http-3A__www.jiscmail.ac.uk_lists_ACB-2DCLIN-2DCHEM-2DGEN.html&d=AwIFAg&c=SgMrq23dbjbGX6e0ZsSHgEZX6A4IAf1SO3AJ2bNrHlk&r=_4rCeX3TSQgrdhhwEl4yDs13b8mZhuDoiRSP4oKKuU4&m=AOWGrF-KHy8M7WIW5Km5Z9khIGSuCxq_XCYo4Zjn1Ms&s=cMxp_NanXU8H1bMHft0ynXNqWBO9iOXZ39XRyBZ8egc&e=
List Instructions (How to leave etc.)
https://urldefense.proofpoint.com/v2/url?u=http-3A__www.jiscmail.ac.uk_&d=AwIFAg&c=SgMrq23dbjbGX6e0ZsSHgEZX6A4IAf1SO3AJ2bNrHlk&r=_4rCeX3TSQgrdhhwEl4yDs13b8mZhuDoiRSP4oKKuU4&m=AOWGrF-KHy8M7WIW5Km5Z9khIGSuCxq_XCYo4Zjn1Ms&s=rZZbQTBaSsZV_YDduI9RBB11sTw04YoR2N1dzUqzMag&e=
------ACB discussion List Information--------
This is an open discussion list for the academic and clinical community working in clinical biochemistry.
Please note, archived messages are public and can be viewed via the internet. Views expressed are those of the individual and they are responsible for all message content.
ACB Web Site
http://www.acb.org.uk
Green Laboratories Work
http://www.laboratorymedicine.nhs.uk
List Archives
http://www.jiscmail.ac.uk/lists/ACB-CLIN-CHEM-GEN.html
List Instructions (How to leave etc.)
http://www.jiscmail.ac.uk/
|
