Dear Mani,

This looks exactly like a bug in ctffind4 that was fixed in versions 
4.0.15 onwards. This bug only affected cases where many instances of 
ctffind4 were run simultaneously in the same folder. The "quality" of 
the results (when ctffind4 ran to completion) was not affected by this bug.

See details in this thread of the ctffind support forum:
http://grigoriefflab.janelia.org/node/4800

So I would recommend upgrading to at least 4.0.15. Sources & binaries 
for linux are available here: http://grigoriefflab.janelia.org/ctf

Best regards,
Alexis


-- 
Alexis Rohou

Research Specialist
Grigorieff Lab
http://grigoriefflab.janelia.org
Tel. +1 571 209 4000 x3485



On 09/21/2015 10:26 AM, Manikandan KARUPPASAMY wrote:
> Hi All,
>
> ctffind4 gives error when run from relion1.4 (SBgrid).
>
> relion 1.4
> ctffind4 : 4.0.13
>
> We run as below from the command line.
> -------------------------
> $mpirun -n 20 `which relion_run_ctffind_mpi` --i "2d_selected.star" 
> --o "micrographs_ctf.star" --ctfWin -1 --CS 2.7 --HT 300 --AmpCnst 0.1 
> --XMAG 140000 --DStep 14 --Box 512 --ResMin 30 --ResMax 5 --dFMin 5000 
> --dFMax 50000 --FStep 500 --dAst 100 --ctffind_exe 
> "/programs/x86_64-linux/ctffind4/4.0.13/bin/ctffind --omp-num-threads 
> 1 --old-school-input"
>
>
>  Estimating CTF parameters using Niko Grigorieff's CTFFIND ...
> 000/??? sec ~~(,_,"> [oo]forrtl: No such file or directory
> forrtl: No such file or directory
> forrtl: No such file or directory
> forrtl: No such file or directory
> forrtl: No such file or directory
> forrtl: No such file or directory
> forrtl: No such file or directory
> forrtl: No such file or directory
> forrtl: No such file or directory
> forrtl: No such file or directory
> forrtl: severe (28): CLOSE error, unit 20, file "Unknown"
> Image              PC                Routine Line        Source
> ctffind            000000000156F4FF  Unknown Unknown  Unknown
> ctffind            00000000017CA31F  Unknown Unknown  Unknown
> ctffind            0000000001746992  MAIN__ 372  ctffind.f90
> ctffind            0000000000400566  Unknown Unknown  Unknown
> ctffind            00000000016C1DFB  Unknown Unknown  Unknown
> ctffind            0000000000400429  Unknown Unknown  Unknown
> forrtl: severe (28): CLOSE error, unit 20, file "Unknown"
> -------------------------
>
> However, this works with 'mpirun -n 1' (no mpi). Are there solutions 
> to fix this?.
>
> thanking you.
>
> with kind regards
>
> Mani.