Dear all,
sorry for the off-topic question but I would like to assess a putative
adenylate cyclase / guanylate cyclase activity in a protein with
proposed 'moonlighting activities'. In our hands and assays, the protein
behaves as a typical protein kinase, but there are several reports about
a guanylate cyclase activity. Thus far, we have checked ATP and GTP
hydrolysis by HPLC and analyzed the products. We could find ADP as
expected for a kinase and small amounts of AMP, but no cAMP. Same for
GTP hydrolysis, which however is not as good a substrate, as one would
expect for a kinase. I would now like to verify these results with an
independent method. Could someone recommend a robust assay for guanylate
and adenylate cyclase activty, that relies on detection of the product?
What would be the 'gold standard' here?
thanks a lot!
Michael
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