here is a nice and simple method on how to select heavy atoms for
phasing using native PAGE (please see Fig. 1):
Structure. 2000 Jul 15;8(7):R143-9.
Screening for phasing atoms in protein crystallography.
Boggon TJ, Shapiro L.
Hope that helps,
On Mon, Jun 29, 2015 at 8:47 AM, Giulliana Rangel
<[log in to unmask]> wrote:
> Dear all,
> I'm looking for a method to solve the phase problem. Thus, I would like some
> help about heavy-atom derivatives.
> What is the best heavy-atom to make soaking? I tried Iodine (because my
> protein have a lot of tyrosines), but no results...
> I read some papers about quick-soak, its a good method? Using platine, Hg,
> Any suggestions? Thank you so much.
> Best wishes,
> Giulliana Rangel
> M.S. Biotechnology Program - UNIFESP
> Structural Biology Laboratory
> Phone: +55 (12) 3309-9698
> 330 Talim St
> ZIP CODE 12231-280
> São José dos Campos - SP/ BR