Hi Linda,
You can use relion_project to do this: relion_project --i volume.mrc --ang
angles.star
The star file you can make yourself with rlnAngleRot, rlnAngleTilt and
rlnAnglePsi values that you want.
Whatever program you use, it is important that the projections maintain
the correct grey-scale, just like relion_reconstruct would do in the other
direction. Jose-Miguel: do Xmipp3 projections do that?
HTH,
S
> Hello Everybody,
> In order to increase my data and improve picking I would like to use my
> current 3D model as a reference for picking an extra dataset. Is it
> possible to do this directly? or if not, can someone explain how to create
> 2d projections from the model at fixed angles in all directions? I know
> the
> latter is an option in Imagic, but we do not have access to that program.
> Thank you.
>
> Best wishes,
> Linda
>
> On Sat, May 9, 2015 at 9:23 AM, Sjors Scheres <[log in to unmask]>
> wrote:
>
>> Hi Joe,
>> This is caused by errors in the MPI messages: perhaps network failures
>> or
>> bugs on the network cards.
>> However, as long as you have the files you mention, you're lucky and
>> this
>> is rescuable. :-)
>> Use relion_reconstruct -- debug --debug_ori_size 200 --debug_size 200
>> --i
>> run1_half1
>> (where run1 is the rootname of your run, and 200 is the size of your
>> images. Do this also for half2. Save the resulting maps with the same
>> unfil.mrc map as you would normally get and then proceed with
>> postprocessing as usual.
>> HTH,S
>>
>>
>> > Hi all,
>> >
>> > I used relion-1.3 to carry out the structure determination of my
>> protein.
>> > However, at the end of auto-refine, the job stopped. Instead of
>> getting
>> > the 3D models from half1 or half2, I got these files:
>> >
>> > _half2_class001_data_real.mrc
>> > _half1_class001_data_real.mrc
>> > _half2_class001_data_imag.mrc
>> > _half1_class001_data_imag.mrc
>> > _half2_class001_weight.mrc
>> > _half1_class001_weight.mrc
>> >
>> > The error code is like:
>> > MPI_ABORT was invoked on rank 2 in communicator MPI_COMM_WORLD with
>> > errorcode 1.
>> >
>> > I tried to run this on either a cluster or a multi-core desktop, it
>> always
>> > stopped at the same point.
>> >
>> > Any suggestion will be much appreciated.
>> >
>> > Kind regards,
>> > Joe
>> >
>>
>>
>> --
>> Sjors Scheres
>> MRC Laboratory of Molecular Biology
>> Francis Crick Avenue, Cambridge Biomedical Campus
>> Cambridge CB2 0QH, U.K.
>> tel: +44 (0)1223 267061
>> http://www2.mrc-lmb.cam.ac.uk/groups/scheres
>>
>
--
Sjors Scheres
MRC Laboratory of Molecular Biology
Francis Crick Avenue, Cambridge Biomedical Campus
Cambridge CB2 0QH, U.K.
tel: +44 (0)1223 267061
http://www2.mrc-lmb.cam.ac.uk/groups/scheres
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