How long are you cryoprotecting for?
You don't really need more than a few seconds. If I have sensitive crystals i tend to just "swipe" them slowly through the final CP solution and often that works. If I would leave them in the CP solution for more than say 30 seconds the crystals would behave like you describe.
GL Bert
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From: CCP4 bulletin board [[log in to unmask]] on behalf of Faisal Tarique [[log in to unmask]]
Sent: Monday, May 04, 2015 7:43 PM
To: [log in to unmask]
Subject: [ccp4bb] cryo condition
Hello everyone
Can anybody suggest me a cryo condition for a crystal obtained in
MIDAS screen of Molecular Dimension:
G1> 0.1MTris8.0G10.1Mpotassium chloride25% v/vSOKALAN®CP7 0.1MHEPES7.0
G2>0.3Mammonium formate20% v/vSOKALAN® CP 5 0.1MHEPES7.0
Crystals are in beautiful cuboid shaped but all sorts of PEG
combinations and Glycerol formulation failed to prevent it from
cracking and dissolving.
Has anybody faced a similar situation as mentioned above and what
precaution was taken to prevent it from cracking or dissolving.
Your suggestions will be of immense help
Thanks in advance
--
Regards
Faisal
School of Life Sciences
JNU
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