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CCPEM  April 2015

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Subject:

Re: Reconstruct Error

From:

Sjors Scheres <[log in to unmask]>

Reply-To:

Sjors Scheres <[log in to unmask]>

Date:

Thu, 23 Apr 2015 07:09:19 +0100

Content-Type:

text/plain

Parts/Attachments:

Parts/Attachments

text/plain (48 lines)

Hello Sudheer,
Did you low-pass filter the images prior to reconstruction/refinement?
That should strictly be avoided, as it may lead to zero noise estimates at
cetrain frequencies and the problems you report.
HTH,
S

> Hello All,
>
> I keep getting the following error and i cant seem to fix it. Any help
> would be greatly appreciated.
>
>
>
> 8.12/8.12 min
> ............................................................~~(,_,">
>  Maximization ...
> 000/??? sec ~~(,_,">
>    [oo] DIRECT_A1D_ELEM(sigma2, i)=  DIRECT_A1D_ELEM(sigma2, i)=
> 4.41399e-311
> 4.41399e-311
> BackProjector::reconstruct: ERROR: unexpectedly small, yet non-zero sigma2
> value, this should not happen...a
> File: src/backprojector.cpp line: 671
> application called MPI_Abort(MPI_COMM_WORLD, 1) - process 24
> BackProjector::reconstruct: ERROR: unexpectedly small, yet non-zero sigma2
> value, this should not happen...a
> File: src/backprojector.cpp line: 671
> application called MPI_Abort(MPI_COMM_WORLD, 1) - process 6
>  DIRECT_A1D_ELEM(sigma2, i)= 4.41399e-311
>  DIRECT_A1D_ELEM(sigma2, i)= 4.41399e-311
> BackProjector::reconstruct: ERROR: unexpectedly small, yet non-zero sigma2
> value, this should not happen...a
> File: src/backprojector.cpp line: 671
>
>
>
> Thanks,
> Sudheer Molugu. Ph.D


-- 
Sjors Scheres
MRC Laboratory of Molecular Biology
Francis Crick Avenue, Cambridge Biomedical Campus
Cambridge CB2 0QH, U.K.
tel: +44 (0)1223 267061
http://www2.mrc-lmb.cam.ac.uk/groups/scheres

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