Hi Stephen,
we crystallized several proteins in a glove box placed in a room with
air condition. We checked the temperature all the times and found that
is had been quite stable.
For one protein that crystallized only at 4 deg C, we setup the
crystallization plate within the glove box and put it into an gas-tight
plexiglass box (built by our workshop). The box with the crystallization
plate was then incubated at 4degC.
Best, Guenter
> Dear CCP4BBer's
>
> Apologies for the off-topic post, but the CCP4BB seems to be the best place to ask about crystallisation.
>
> I am looking to set up crystallisation in an anaerobic glove box and wondered how other people did this, specifically the crystallisation stage. My initial thoughts were to place a small crystallisation incubator inside the box, however the smallest I have come across so far (~27L) is still rather large. Has anyone come across smaller incubators? Alternatively are incubators even neccessary if the glove box is placed in a room with good air conditioning and stable temperature control?
>
> Any recommendations would be very helpful.
>
> Thanks in advance,
>
> Steve Carr
>
> Dr Stephen Carr
> Research Complex at Harwell (RCaH)
> Rutherford Appleton Laboratory
> Harwell Oxford
> Didcot
> Oxon OX11 0FA
> United Kingdom
> Email [log in to unmask]
> tel 01235 567717
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