We switched to CTFfind4 recently. It worked well. Meanwhile, as showed in
the enclosed png file (pixel 1.05 A), we noticed that there is a circular
mask to ~2.92 angstrom, and beyond that resolution, there were evenly
spaced rings in the background. It does not affect the accuracy of the
fitting. I am curious to know whether anyone in this group noticed the
same and/or tried to get rid of the background. Thanks.
On 3/1/15, 4:10 AM, "Sjors Scheres" <[log in to unmask]> wrote:
>Sometimes jobs die due to glitches in the system. You may then miss your
>star file. The program has an option --only_make_star that you can use in
>this cases: it will only generate the star file, without having to re-run
>the whole job.
>> Hi Sjors,
>> I met a problem recently when I deal with movie data. I followed manual.
>> After ran the CTF estimation, it didn't generate the xxxx_ctf.star file.
>> However, I can find all ctf files in the Micrographs folder with for
>> mrc files, for example, ctffind3.com, and ctffind3.log. I used ctffind4
>> do the ctf correction.
>> Is there a way or command to generate the ctf_star file from the results
>> in Micrographs folder? Why did it happen? How to correct it?
>> The command line I used is shown below
>> /opt/relion/1.3/bin/relion_run_ctffind_mpi --i all_AVG.star --o
>> all_AVG_ctf.star --ctfWin -1 --CS 2 --HT 300 --AmpCnst 0.1 --XMAG 122058
>> --DStep 14 --Box 512 --ResMin 100 --ResMax 7 --dFMin 5000 --dFMax 50000
>> --FStep 500 --dAst 0 --ctffind3_exe /opt/ctffind/4.0.8/bin/ctffind
>> --omp-num-threads 1 --old-school-input"
>> Thanks a lot.
>> You have a wonderful weekend.
>> Sincerely yours,
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