If you've checked there are no all-black particles or the like, I guess
this is OK. Just to be sure: you are scaling down (by a factor 1/2),
right? Scaling up would not make sense and should be avoided.
> Hey Everyone,
> I re-scale my auto-picked particles by a factor of 2 using the rescale
> option in particle extraction and normalize them by indicating 'yes' in
> the Normalize particles option. When I run 2D classification with these
> particles, I get an error message stating that the particles have not been
> normalized. Since I know I normalized my particles in the particle
> extraction step, is it ok to go on with 2d classification using the
> --dont_check_norm option?
> I have had this issue with two different data sets now.
> Best Wishes,
> Ali Khan
MRC Laboratory of Molecular Biology
Francis Crick Avenue, Cambridge Biomedical Campus
Cambridge CB2 0QH, U.K.
tel: +44 (0)1223 267061