Hi Klim,
The particles_movie.star file should also contain CTF values (like
rlnDefocusU etc). Were the ctffind3.log files in the micrographs directory
when you extracted movie frames?
HTH,
S
> Hi,
>
> I was performing the movie expansion as described in the tutorial but ran
> into the problem. Relion reports that there are no CTF values found in the
> star file. I have looked in the resulting _data.star file for the movie
> iteration, and there are no defocus values in it. However, the defocus
> parameters are present in the _data.star file(non movie) which is
> corresponding to the _optimser.star file from which I continued the run
> for movie expansion.
>
> Here is the exact error message:
> CTF::initialise: ERROR: CTF initialises to all-zero values. Was a correct
> STAR file provided?
> File: src/ctf.cpp line: 169
>
> In thread 0
> CTF::initialise: ERROR: CTF initialises to all-zero values. Was a correct
> STAR file provided?
> File: src/ctf.cpp line: 169
>
> In thread 0
> CTF::initialise: ERROR: CTF initialises to all-zero values. Was a correct
> STAR file provided?
> File: src/ctf.cpp line: 169
>
> In thread 0
> CTF::initialise: ERROR: CTF initialises to all-zero values. Was a correct
> STAR file provided?
> File: src/ctf.cpp line: 169
>
>
> Here are the contents of the .out file:
>
> Expanding current model for movie frames...
>  Auto-refine: Iteration= 18
>  Auto-refine: Resolution= 10.52 (no gain for 0 iter)
>  Auto-refine: Changes in angles= 999 degrees; and in offsets= 999 pixels
> (no gain for 0 iter)
>  Auto-refine: Refinement has converged, entering last iteration where two
> halves will be combined...
>  Auto-refine: Angular step= 15 degrees; local searches= false
>  Auto-refine: Offset search range= 3 pixels; offset step= 0.84375 pixels
>  CurrentResolution= 10.52 Angstroms, which requires orientationSampling of
> at least 6 degrees for a particle of diameter 200 Angstroms
>  Oversampling= 0 NrHiddenVariableSamplingPoints= 37
>  OrientationalSampling= 15 NrOrientations= 1
>  TranslationalSampling= 0.84375 NrTranslations= 37
> =============================
>  Estimated memory for expectation step  > 0.230466 Gb, available memory =
> 32 Gb.
>  Estimated memory for maximization step > 0.234839 Gb, available memory =
> 32 Gb.
>  Expectation iteration 18
> 000/??? sec ~~(,_,">
>
> Here is the header for the _data.star file for the movie iteration:
> data_images
>
> loop_
> _rlnImageName #1
> _rlnCoordinateX #2
> _rlnCoordinateY #3
> _rlnMicrographName #4
> _rlnParticleName #5
> _rlnAngleRot #6
> _rlnAngleTilt #7
> _rlnAnglePsi #8
> _rlnOriginX #9
> _rlnOriginY #10
> _rlnAngleRotPrior #11
> _rlnAngleTiltPrior #12
> _rlnAnglePsiPrior #13
> _rlnOriginXPrior #14
> _rlnOriginYPrior #15
> _rlnClassNumber #16
> _rlnRandomSubset #17
> _rlnNormCorrection #18
> _rlnOriginalParticleName #19
> _rlnGroupName #20
> _rlnNrOfFrames #21
>
> Are there supposed to be defocus values present in the _data.star file for
> the movie frames? If so, what could cause for them not to be written
> there?
>
> Thanks,
>
> Klim
>


-- 
Sjors Scheres
MRC Laboratory of Molecular Biology
Francis Crick Avenue, Cambridge Biomedical Campus
Cambridge CB2 0QH, U.K.
tel: +44 (0)1223 267061
http://www2.mrc-lmb.cam.ac.uk/groups/scheres