Hi Sjors and everyone.
I am having issues with 3D refinement in relion1.3. It runs fine until
the last step, producing nice looking 3D half maps.
After the final iteration I get the two _unfil.mrc maps, and they are
not right. Sometimes both of them, sometimes only one, they show weird
streaks, and obviously my final result does not make sense.
This is remarkably similar to what Mike Cianfrocco had reported
previously in this thread, although he solved the issue by increasing
available disk space (temporary needs seem quite high for the last
refinement step). I have >1T of space left (and <2G of data) so that is
not my problem, also memory seems not to be the issue (I have 4G
available and the log file tells me >1.05G are needed for expectation step).
I started having this problem only when I started using a new cluster
for testing. The same dataset runs just fine on our local 48 core
machines. The main difference between the two systems is that our
machines (where refinement works) are AMD and the cluster (where I have
the problem), is Intel.
I should mention the cluster allows 2D and 3D classification to run to
the end with no problems.
Any help with this would be greatly appreciated!
Thanks!
Giulia
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Giulia Zanetti
ISMB, Birkbeck College
Malet St. London
WC1E 7HX
02076316898
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