Dear Kyle,
Supervised classification is possible as discussed previously on this
list. Give a STAR file at the --ref option in addition to the --K
argument.
HTH, S
> Dear Sjors,
>
> Thanks for the previous advice. That’s perfect.
>
> On another note, if I may ask, as well as dealing with interesting
> symmetries I am iteratively 3D classifying a heterogeneous ptcl set, I
> know I have certain architectures and am looking for them. Would there be
> any benefit in getting Relion to use multiple reference volumes in one 3D
> classification and is this possible? Or is my approach of classifying with
> one structure, then taking what's left and classifying against the other
> structure a valid method?
>
> Thanks again for the support.
>
> Best,
> Kyle
>
> On 12/01/15 15:47, Sjors Scheres wrote:
>> Dear Kyle,
>> Not sure this would be a good idea. But if you want to change the
>> symmetry, then you need to change and recompile the code. The files are
>> in
>> src/symmetries.h and src/symmetries.cpp
>> HTH, S
>>
>>> Hi everyone,
>>>
>>> I would like to add a new symmetry group for use in autorefine in
>>> Relion
>>> 1.3. Could anyone indicate where I should set up the file to be
>>> referenced?
>>>
>>> Many thanks in advance,
>>> Kyle
>>>
>>> rot_axis 3 0 0 1
>>> rot_axis 3 0 0.577350 -0.204124
>>>
>>>
>>
>
--
Sjors Scheres
MRC Laboratory of Molecular Biology
Francis Crick Avenue, Cambridge Biomedical Campus
Cambridge CB2 0QH, U.K.
tel: +44 (0)1223 267061
http://www2.mrc-lmb.cam.ac.uk/groups/scheres
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