Hi Ludo,
My suggestion is that in any case your boxsize should be at least 1/3
larger, especially if your particle are not CTF corrected. One thing for
the benefit of larger box is that there is a lot of information around
your particles because of CTF modulation in Fourier space. Even if your
particles are already CTF corrected, FFT introduces artifact if the box
is too small.
Kai
On Mon, 2014-12-15 at 11:15 +0000, Ludovic Renault wrote:
> Hi all,
> I was wondering if like in eman2 there were any better box sizes to
> get faster processing? Especially for the 2d classification step which
> is the bottleneck really.
> Just out curiosity, is adding 1/3 of particle diameter to your box is
> a good consensus in relion? 250 ang particle diameter with a box of
> approx. 330 ang diameter for example?
> Thanks,
> Ludo
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