Hi,
Can you please reply with a quick step by step procedure for multirefine.
Thanks,
Sudheer.
Sent from my iPhone
> On Nov 23, 2014, at 2:43 AM, Sjors Scheres <[log in to unmask]> wrote:
>
> Hi Scott,
> Both scenarios can be dealt with in RELION, although the multi-reference
> 3D refinement will typically be more successful in scenario i) than in
> scenario 2).
> HTH, S
>
>> To follow on, it would appear two distinct scenarios can be discussed
>> here: i) a complex with/without ligand, i.e., multiple DEFINED
>> conformations; and ii) an inherently flexible molecule with a CONTINUUM of
>> different conformations (which is what I'm dealing with in EMAN).
>>
>> I assume RELION can handle both scenarios. Delving into the RELION
>> tutorial is high on my to-do list - is there an opportunity to explore the
>> conformational heterogeneity issue in the tutorial?
>>
>> Regards,
>> Scott
>>
>>
>>
>> Hi,
>> A similar question has been asked a few days ago.
>> I copy the thread below but you can also search archives on the ccpem
>> mailing list website: https://www.jiscmail.ac.uk/cgi-bin/webadmin?A0=ccpem
>>
>> Ludo
>> -----------
>> -----------
>> Hi all,
>>
>>
>> When we are talking about using the 3D classification to distinguish the
>> complex with/without ligand, could we introduce two different initial
>> model (with/without ligand) as references to relion 3D classification?
>>
>>
>>
>>
>> -----Original Message-----
>> From: Collaborative Computational Project in Electron cryo-Microscopy
>> [mailto:[log in to unmask]] On Behalf Of Molugu, Sudheer kumar
>> Sent: Friday, 21 November 2014 12:31 PM
>> To: [log in to unmask]
>> Subject: [ccpem] multi model refinements in RELION
>>
>> Hello,
>>
>> I was wondering if RELION can handle multimodel refinements. My data set
>> is heterogeneous and a mixture of three conformations. I am currently
>> using multirefine program in EMAN for my 3D model refinements. I would be
>> glad to use RELION if there is an inbuilt algorithm for refining more than
>> one model paralellely from the same raw data set.
>>
>> Thank you very much for your time.
>>
>> Best,
>> Sudheer Molugu Ph.D
>> cryoEM Facility Manager
>> Dept. of Chemistry
>> The University of Texas at El Paso
>
>
> --
> Sjors Scheres
> MRC Laboratory of Molecular Biology
> Francis Crick Avenue, Cambridge Biomedical Campus
> Cambridge CB2 0QH, U.K.
> tel: +44 (0)1223 267061
> http://www2.mrc-lmb.cam.ac.uk/groups/scheres
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