Hi Gang,
Perhaps you could just phase-flip the micrographs prior to particle
extraction? If you do this, don't forget to indicate that your data have
been phase-flipped in subsequent refinements.
HTH,
S
On 11/19/2014 10:46 AM, Gang Cai wrote:
> Hello all,
>
> We wish to use the phase flipped particles for 2D/3D classfication. I am
> wondering how to extract particles with the phase flipped. Many thanks,
> in advance!
>
> Best,
>
> Gang
--
Sjors Scheres
MRC Laboratory of Molecular Biology
Francis Crick Avenue, Cambridge Biomedical Campus
Cambridge CB2 0QH, U.K.
tel: +44 (0)1223 267061
http://www2.mrc-lmb.cam.ac.uk/groups/scheres
|