Hi Manjalu,
In my opinion, one way is to use iEX column. Depending of the Pi of you protein and the one of the BSA, if they are sufficiently different, Ion exchange is on possible way.
To separate your protein from BSA, you have to give us more information about your protein if it's possible.
HTH.
Nicolas
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De : CCP4 bulletin board [[log in to unmask]] de la part de Manjula Ramu [[log in to unmask]]
Envoyé : mercredi 8 octobre 2014 15:27
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Objet : [ccp4bb] How to separate BSA present in Thrombin
Hi all,
My protein size is nearly the same size of BSA and it has His tag. If I have to cleave the tag I have to use Thrombin which is having huge amount of BSA in it. Now how will I separate this BSA from my protein.
Thanks and Regards,
Manjula R
Research Scholar
Department of Biophysics
National Institute of Mental Health and Neurosciences
Bengaluru-29, Karnataka
INDIA
E-mail: [log in to unmask]<mailto:[log in to unmask]>
Mobile no:+91-9538553356
http://www.nimhans.kar.nic.in/
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