Dear Herman,
Assuming that the protein is eukaryotic, and glycans N-linked, various ways of dealing with the issue are described in PMID: 17355862.
Best wishes,
radu
------------------------------------------
A. Radu Aricescu, PhD
MRC Senior Research Fellow
Associate Professor
University of Oxford
Wellcome Trust Centre for Human Genetics
Division of Structural Biology
Roosevelt Drive, Oxford OX3 7BN
United Kingdom
Phone: +44-1865-287564
Fax: +44-1865-287547
https://www.strubi.ox.ac.uk/research/a-radu-aricescu
---- Original message ----
>Date: Wed, 15 Oct 2014 13:03:13 +0000
>From: CCP4 bulletin board <[log in to unmask]> (on behalf of [log in to unmask])
>Subject: [ccp4bb] Trimming of carbohydrate chains
>To: [log in to unmask]
>
>Dear Bulletin Board,
>
>I am struggling with a protein domain of 15 kDa, with about 22 kDa carbohydrate attached. So far, the domain did not crystallize and I suspect the carbohydrate may hinder crystallization. Completely removing the carbohydrate results in low expression yields and poorly soluble protein, so I would like to try to remove some, but not all carbohydrate. Does anyone has a good protocol to trim, but not completely remove the carbohydrate?
>
>Thank you,
>Herman Schreuder
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