Dear ravi kumar,
I would not use the refined mtz-file, but the original data and run phaser with
one model in fixed position, searching for a second copy.
Best,
Tim
On Sat, Sep 13, 2014 at 10:32:10AM +0530, Reddiravikumar Kumar wrote:
> Hi,
>
> I am working on a protein with a resolution of 2.8 A, I have scaled it and
> got solution of R-work and R-free of 0.38 and 0.43 by using 33% identical
> protein as a model. My protein exits as a dimer and crystallized the dimer.
> In solution I got only one molecule, i could see 80 % of sequence is
> matching with electron density also Mathew's coefficient says it is two
> molecules. How can I kept the previously modeled one and search for other
> molecule by using the refined mtz and pdb files?. Are there any programs in
> ccp4suite. please suggest me.
> --
> ravi kumar
--
--
Dr Tim Gruene
Institut fuer anorganische Chemie
Tammannstr. 4
D-37077 Goettingen
GPG Key ID = A46BEE1A
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