Dear Faisal,
at 2.6A resolution I would not call 23A^2 and 24A^2 'a difference',
rather a fluctuation. Parameters are not totally independent from each
other and not from experimental issues.
It most likely also make a difference which program you use. Some
program has a upper cut-off of 100-200 (which I think is reasonable)
while I have seen atoms in the PDB with B-values in the region of 500A^2
(which is close to saying the resolution of the data set is less than
5A...).
Best,
Tim
On 08/14/2014 10:30 AM, Faisal Tarique wrote:
> Dear all
>
> I request you to please tell me the difference between wilson B-factor and
> average B, all atoms ?? I have two structures one native at 2A resolution,
> having mean b factor of 24 and the other a complex structure of the same
> protein with a ligand (at 2.6A resolution) has mean b factor of 23..Why
> there is a difference in the b factor for these two coordinates with the
> later having (low resolution) lower value than the previous (high
> resolution)..?? is this a normal phenomenon or the stability is due to
> bound ligand molecule, which is stabilizing the whole structure ?? Please
> pour some light in this direction..
>
--
Dr Tim Gruene
Institut fuer anorganische Chemie
Tammannstr. 4
D-37077 Goettingen
GPG Key ID = A46BEE1A
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