Hi Marty,
This is very dangerous. It can work, but you will have to change the
numbering of the particles in the STAR file. Look here for the exact
definition of it:
http://www2.mrc-lmb.cam.ac.uk/relion/index.php/Conventions_%26_File_formats#Metadata_I.2FO
Probably, things would be easier if you'd stay inside RELION. ;-)
HTH,
S
On 07/11/2014 04:30 PM, Martin Smith wrote:
> Hi,
>
> We are currently running relion 1.3 with stacks that have been
> modified. Ocassionally, the programs we are implementing will remove
> a particle that is too close to the edge of a micrograph from the
> .mrcs file. However, the starfiles that relion uses from each
> micrograph still contain a link to the particle that has been
> removed. That is, the starfile will have information for 5 particles
> from the micrograph, but the updated .mrcs file will only contain 4
> updated particles. Relion seems to be okay with this situation (i.e.
> no errors). However, I was wondering how relion is deals with the
> problem and why it is okay. Might this stuation lead to any obvious
> problems to consider for the future.
>
> Thanks,
> Marty
--
Sjors Scheres
MRC Laboratory of Molecular Biology
Francis Crick Avenue, Cambridge Biomedical Campus
Cambridge CB2 0QH, U.K.
tel: +44 (0)1223 267061
http://www2.mrc-lmb.cam.ac.uk/groups/scheres
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