Why not draw out a pasteur pipette using a bunsen burner to the desired thinness? You’ll end up with something rather like an old-fashioned glass capillary.
Adrian
On 7 Jul 2014, at 17:52, Matthew Franklin <[log in to unmask]> wrote:
> Hi Frank -
>
> How about a gel loading pipet tip as a substitute for the quartz capillary? Suck the crystal into it, then try to get it to stick to the wall. Flame seal the tip end, and use a glob of vacuum grease for the other end (or cut off the skinny part with your crystal using a razor blade).
>
> That semi-transparent plastic FPLC tubing (Tefzel?) might work as a substitute for the Mitegen capillary sleeve.
>
> Your Xray absorption and background scattering will be really high from all this plastic, but any port in a storm.
>
> - Matt
>
>
> On 7/7/14 12:32 PM, Frank von Delft wrote:
>> Hi all
>>
>> Pretend you were stuck having to do RT data collection but without access to either Mitegen MicroRT Capillaries or the more old-fashioned quartz capillaries, to pop over the loop.
>>
>> Anybody have suggestions of alternative ways of doing this? I do want to use loops (I never learnt how to suck up crystals in capillaries).
>>
>> I have access to a passably stocked biochemistry teaching lab, and could at a pinch go rifle some more advanced research labs. (No, I'm not at home ;)
>>
>> Thanks!
>> phx
>>
>>
>
>
> --
> Matthew Franklin, Ph. D.
> Senior Scientist
> New York Structural Biology Center
> 89 Convent Avenue, New York, NY 10027
> (212) 939-0660 ext. 9374
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