Dear Appu,
If it is possible to solve your structure with currently available MR models using any currently existing technology, one of these programs will be able to do it. Throwing yet another program at the problem won’t help, especially an older program that was good in its time (and is still, to be fair, cutting edge for some things) but is not up-to-date with current molecular replacement algorithms.
Some structures can’t be solved by molecular replacement. We can now do a better job of predicting which ones will be very hard, and this is given in the expected LLG output of Phaser. If that says that the problem is expected to be very difficult, you haven’t made any mistakes in running the programs, and you’ve tried all the strategies recommended by the authors of those programs, then your efforts will be better spent in trying to grow better crystals or doing experimental phasing.
Best wishes,
Randy Read
On 12 Jun 2014, at 20:14, Appu kumar <[log in to unmask]> wrote:
> Hello All,
> I have tried MR in Phaser, MRage, Molrep , Mrbump but i am not getting the true solution which it supposed to be. Although the resoution of data is 6.1A, but i want to give a try to CNS and see if I can find right solution. I have read the manual of CNS but i am unable to get the CNS running. Would you please help me in running the CNS program.
>
> Thank you
> Appu
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Randy J. Read
Department of Haematology, University of Cambridge
Cambridge Institute for Medical Research Tel: + 44 1223 336500
Wellcome Trust/MRC Building Fax: + 44 1223 336827
Hills Road E-mail: [log in to unmask]
Cambridge CB2 0XY, U.K. www-structmed.cimr.cam.ac.uk
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