Dear Shikha,
Such high resolution in the 3rd iteration sounds strange. Are you
low-pass filtering the initial model as much as you can? E.g. say 30-40 A
for such a small protein? That is highly recommended to prevent a bias
towards an incorrect initial model.
HTH,S
> Hello All,
> I am refining a structure of 350kDa protein in relion.
> Weired thing which i noticed is, that resolution increased to 7.7A in 3rd
> iteration and then goes back to 11.1A in next iteration and never come
> back
> to 7 A. My question is this is normal to refinement? I have never faced
> this issues before but this time it giving me large variation in
> resolution.
>
> I will be highly thank you for your help and suggestion
>
> --
> Shikha Singh
> Research Scholar
> c/o Dr. Ashish
> Protein Science and Engg. Div.
> IMTech, Chandigarh
>
--
Sjors Scheres
MRC Laboratory of Molecular Biology
Francis Crick Avenue, Cambridge Biomedical Campus
Cambridge CB2 0QH, U.K.
tel: +44 (0)1223 267061
http://www2.mrc-lmb.cam.ac.uk/groups/scheres
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