Dear Tassos,
Do you know by chance whether BLEND is available?
Best wishes,
Alex
On 27 Mar 2014, at 16:06, Tassos Papageorgiou <[log in to unmask]> wrote:
> Hi,
>
> You may also try BLEND to choose the optimal data sets before scaling and merging
>
> Foadi J, Aller P, Alguel Y, Cameron A, Axford D, Owen RL, Armour W, Waterman DG, Iwata S & Evans G (2013) Clustering procedures for the optimal selection of data sets from multiple crystals in macromolecular crystallography. Acta Crystallogr D Biol Crystallogr 69: 1617–1632
>
> Tassos Papageorgiou
>
> Jarrod Mousa wrote:
>> Hi,
>> I am trying to solve the structure of a membrane protein. The protein has 12 helices and I have a good molecular replacement model that seems to work for about half of the structure. I used chainsaw to convert the amino acid residues to that of my protein sequence, and the density fits the structure well on one side of the protein, but on the other side (about 5 helices), there doesn't seem to be any density for the side chains. Has anyone had experience with this? The completeness is high ~99% for 3.2 angstroms. The data was collected from fairly small crystals ~ 20um.
>> Thanks.
--
Alex Batyuk
The Plueckthun Lab
www.bioc.uzh.ch/plueckthun
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