A lot of "ageing" sonicators are not really ageing. Have you tried cleaning the tip of the sonicator? If not, remove the tip from the sonicator and use a 800 Grit wet sandpaper to make it as shiny as it was when you first bought it. I do this every three times I use the sonicator. A lot of crap deposits on it with every use.
Best wishes,
Reza
Reza Khayat, PhD
Assistant Professor
The City College of New York
Department of Chemistry, MR-1135
160 Convent Avenue
New York, NY 10031
Tel. (212) 650-6070
---- Original message ----
>Date: Tue, 4 Feb 2014 12:40:51 -0500
>From: CCP4 bulletin board <[log in to unmask]> (on behalf of Roger Rowlett <[log in to unmask]>)
>Subject: Re: [ccp4bb] off-topic: bug busting
>To: [log in to unmask]
>
> BeadBeater. http://biospec.com/. Gentle,
> aerosol-free way to break 15-350 mL of cell paste
> (2-150 g wet packed cells).
>
> Cheers,
>
> _______________________________________
> Roger S. Rowlett
> Gordon & Dorothy Kline Professor
> Department of Chemistry
> Colgate University
> 13 Oak Drive
> Hamilton, NY 13346
>
> tel: (315)-228-7245
> ofc: (315)-228-7395
> fax: (315)-228-7935
> email: [log in to unmask]
>
> On 2/4/2014 11:49 AM, Phoebe A. Rice wrote:
>
> Some time ago, there was a nice discussion of
> cost-effective, wimpy protein-friendly ways to
> break open E. coli. We're thinking about
> replacing an aging sonicator. If people have a
> favorite gizmo, could they repeat that advice?
> thank you,
> Phoebe Rice
>
> ++++++++++++++++++++++++++++++++++++++++++
>
> Phoebe A. Rice
> Dept. of Biochemistry & Molecular Biology
> The University of Chicago
>
> 773 834 1723; [log in to unmask]
> http://bmb.bsd.uchicago.edu/Faculty_and_Research/
>
> http://www.rsc.org/shop/books/2008/9780854042722.asp
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